عنوان :
آيا منشاء
سلولهاي CLL همان - B
لنفوسيتهاي CD 5 + است ؟
نويسندگان
: دكتر منصور
صالحي1 ،
دكتر رسول
صالحي1 ،
دكتر
عبدالرضا
صباحي2 ،
پروفسور
مالكو
م
گوينز 3
1 گروه
ژنتيك و
بيولوژي
مولكولي ، 2 گروه
علوم تشريحي –
دانشكده
پزشكي –
دانشگاه علوم
پزشكي
اصفهان
- كدپستي 176 – 81744
3 انستيتو
تحقيقات
سرطان ، دانشگاه
شفيلد ،
انگلستان
باور
عمومي بر اين
است كه B
لنفوسيتها
معادل غير
سرطاني
سلولهاي B در CLL هستند
علت اين باور
مرفولوژي اين
سلولها و
ايمنوگلوبولينهاي
موجود در سطح
آنها مي باشد .
اكثر سلولهاي CLL
ماركرهاي
منحصر به
سلولهاي B - lineage را بيان مي
كنند ولي
تفاوتهائي
نيز بين
سلولهاي CLL
و B - Cell ها نيز
يافت شده است .
دراين
مطالعه از ژل
الكتروفورز 2 - D براي
مطالعه ميزان
شباهت در بيان
پروتئينها
بين سلولهاي CLL و
لنفوسيتهاي
غير سرطاني
مورد استفاده
قرار گرفت .
نتايج حاصل به
وضوح نشان
دادند كه سلولهاي
CD5+ , B
شبيه ساير
لنفوسيتهاي B بوده و
با سلولهاي CLL كاملا
متفاوت اند .
گر
چه اطلاعات
ارائه شده در
اين مقاله
منشاء سلولهاي
CLL
را مشخص نمي
كند ولي
احتمال اينكه
اين سلولها از
لنفوسيتهاي CD5+ , B
خون محيطي
منشاء گرفته
باشد را رد مي
كند .
TITLE :
Is the origin of CLL cells CD+B-lymphosytes?
AUTHOR: M.salehi ,
r.salehi ,.a.r.sabahi and M.H.Goyns
Dept .
of Genetics and molecular biology , dept . of anatomical sciences medical school . Isfaha
Institute of cancer studies , medical school , sheffield
university sheffield ,
Criculating
B-lymphocytes are traditionally thought to be the non-malignant counterpart of
B-cells CLL because of their morphology and expression of surface immunoglobulins
. The majority of CLL cells express B-lineage restricted markers , but some differences between CLL and normal
B-cells has also been found .
In this study 2D-gel
electrophoresis were used to study similarity between protein expression id CLL
cells and non-malignant lymphocyts . Our results clearly demonstrates that CD5+B-cells were
similar to other B-lymphocytes and quite unlike CLL is derived , but rather
cast doubt over the possibility that it originated from the CD5+B-lymphocytes
in the peripheral blood .
TITLE :
Studies on the possible changes in Haematological
parameters of RBCs in the Blood Unites during the
period of storage in the
Part I :
Changes in the RBC Enzyme G6PD .
Dr. Chitnis , P. & Dr.Imam ,S,J .
Histology Dept ., Medical college , A.U.M.S & blood bank center ,
Abstract
:
In the blood bank centers , standard procedures are followed for the proper
collection storage and distribution of Blood units . during
this period of storage in the CPD bags
possible changes may occur in the various components of Blood which might attect the quality of the blood units for safe trasfusion therapy . few studies
on this problem are reported . hence work is undretaken to study the morphological , physiological , and
metabolic chages in the RBCs
during the storage period .
G6PD is one of the
important RBC enzyme and its defect (deficiency ) is
associated with haemolytic anaemia
, which affects many individuals . hence it is
important to know the possible dhanges in this enzyme
during the storage . In normal RBCs the stability and
catalytic activity of the enzyme G6PD are slowly degraded over the life span
(120 days ) of RBCs . The
primary metobolic role of the enzyme G6PD in the
pentose phosphate pathway is to generate NADPH which helps to maintain glutathion in reduced state for-anti- oxidant activity .
The blood units (bags with
CPD) are randomly selected , marked andkept along with other units in the storage room (4 C) . samples for analysis are taken as follows ; (1) blood of
Donors (outside bags ) to be used as control (base values ) . (2) blood samples on the 1 day and later on every 5 days , till
the expiry date (28 days ) of storage . Thus 20 untis
and 8 samples from each are studied .
For the evaluation of G6PD
activity , procedures of sigma kit (U.S.A) is followed
. The results are tabulated for 20 randomly selected Donors and their blood units . Mean values for 20 units (bags) over each subsequent
5-days-evaluation show that there is a rapid decline in G6PD activity from the normal ( 1 day –
26.7 mins ) to partial deficiency ( 10 day – 50 mins ) . and almost gross
deficiency ( 28 day – 90 mins ) of G6PD enzyme in RBCs . Again it is noticed that 3 of the 20 donors (15%)
are found to be G6PD gross deficient subjects , prior
to blood collection (i.e.outside the bags )
The significance of this
study and its implication for jthe safe Transfusion
therapy especially in conditinon of haemolytic anaemia , is explained . it is recommended
that in areas of high prevalence of G6PD
deficiency ( or favism ) donors shuld be routinelyscreened for
G6PD enzyme status and only fresh blood
should be given to G6PD dificient patients in haemolytic crisis .
Title :
Donor mononuclear cell Infusion for the treatment of relapse after allogeneic marrow transphantation
in beta thalassemia major .
Authors
: Dr.sh.keyhanian , A.Ghavamzadeh
, M jahani , b.bahar , k.alimoghaddam , SH.Key hanian . s.gholibeikian .
Hematology –Oncology &
BMT Research center of
We have used donor mononuclear cell infusion for treatment of relapse after bone marrow trasplantation in two patients with beta thalassemia major . Two trasfusion dependent patients with unstable mixed chimerism after BM transplantations were treated with Donor Mononuclear cell infusion . one of them was a 4 years old boy with thalassemia major class I , which received 3.5 × 108/kg cells 8 months after transplantation and the other one was a 6 years old boy with thalassenia major class III , received 4× 108 / kg cells 7 months after transphantation . At present , the first patient is transfusion independent with stable Hb level but also a sever GVHD of skin (grade IV) .The second patients received second transplantation (PBSC) 7 months after Donor mononuclear cell Infusion without engraftment and he is trasfusion dependent .